ngµç×ÓÓÎÏ·ÉúÃü¿ÆÑ§Ñ§ÔºÂæ¹ÛÕý½ÌÊÚÍŶÓÌá³öÒ»ÖÖÓÃÓÚÅжÏ΢Á¿ÑùÆ·m6AµÄ¸ßЧ±ã½Ý½¨¿â²âÐòÊÖÒÕ£¬£¬Åжϵ½Êýǧ¸ö¸ßÖØ¸´ÐÔm6Aλµã£¬£¬ÎªÔö½øm6A¹¦Ð§Ñо¿ÌṩҪÁìѧÁ¢Òì¡£¡£¡£
N6-¼×»ùÏÙàÑßÊ(N6-methyladenosine, m6A) ÊÇÕæºËÉúÎïmRNAÖк¬Á¿×ºñµÄ¼î»ùÐÞÊÎÀàÐÍ£¬£¬¶¯Ì¬µ÷¿ØmRNAµÄ¼ô½Ó¡¢¡¢¶¨Î»¡¢¡¢½µ½âµÈ¶àÖÖ´úлͨ·£¬£¬½ø¶ø¼ÓÈëϸ°û·Ö»¯¡¢¡¢Ö×Áö±¬·¢¡¢¡¢ÃâÒß·´Ó³µÈÖ÷ÒªÐÄÀíÀú³Ì¡£¡£¡£¼×»ù»¯RNAÃâÒß¹²³Áµí¸ßͨÁ¿²âÐò(MeRIP-Seq »òm6A-Seq) ÊÇÏÖÔÚÓ¦ÓÃ×îÆÕ±éµÄm6A¼ì²âÊÖÒÕ£¬£¬µ«¸ÃÊÖÒÕͨ³£ÐèÒª´ó×ÚÆðʼRNAÑùÆ·£¨>100¦Ìg×ÜRNA£©£¬£¬ÄÑÒÔÓ¦ÓÃÓÚ΢Á¿ÑùÆ·µÄm6A¼ì²â£¬£¬ÏÞÖÆÁËÆäÔÚÁÙ´²Ï¡È±ÑùÆ·ºÍÔçÆÚÅßÌ¥·¢ÓýµÈ³¡¾°ÖпªÕ¹m6AÑо¿¡£¡£¡£Òò´Ë£¬£¬¸ÃÁìÓò¼±ÐèÒ»ÖÖÊÊÓÃÓÚµÍÆðʼRNAÁ¿¡¢¡¢¼òÆÓ¿ì½ÝµÄm6A¸ßͨÁ¿²âÐòÊÖÒÕ¡£¡£¡£
¸ÃÑо¿Ìá³öÒ»ÖÖm6A¸ßͨÁ¿²âÐòÐÂÊÖÒÕ£º£º£ºtMeRIP-Seq¡£¡£¡£¸ÃÊÖÒÕͨ¹ý¶à¾ÛÐØÏÙà×à¤ÒýÎïÌØÒìÐԵط´×ªÂ¼º¬ÓÐPolyAβµÄmRNA£¬£¬Ê¹ÓÃTn5ת×ùøÇиîRNA/DNAÔÓºÏÁ´µÄÌØÕ÷£¬£¬½«mRNA/DNAÔÓºÏÁ´Æ¬¶Ï»¯Í¬Ê±¼ÓÉϲâÐòÌÖÂÛ£¬£¬¼«´óµØ½µµÍÑùÆ·³õʼͶÈëÁ¿²¢¼ò»¯ÊµÑé²Ù×÷¡£¡£¡£»£»£»ùÓÚÕâÒ»Õ½ÂÔ£¬£¬¸ÃÑо¿ÒÔµÍÖÁ60ng×ÜRNAΪÆðʼģ°å£¬£¬Åжϵ½Êýǧ¸ö¿ÉÖØ¸´m6Aλµã£¬£¬ÕâЩλµãÌØÒìµØ¸»¼¯ÓÚ»ùÒò3¡ä×îºóÖÜΧ£¬£¬²¢ÇÒÓе䷶µÄRRACH»ùÐò¡£¡£¡£Ö®ºóÑо¿ÕßÓ¦ÓøÃÊÖÒÕ´ÓÒ»µÎѪÖÐÅжÏÁËm6AͼÆ×£¬£¬·¢Ã÷¶à¸öѪҺ¼²²¡Ïà¹Ø»ùÒò¿ÉÄÜÊÜm6Aµ÷¿Ø¡£¡£¡£»£»£»ùÓÚÀàËÆÔÀí£¬£¬tMeRIP-SeqÊÖÒÕδÀ´»¹¿ÉÄÜÍØÕ¹µ½ÆäËüRNAÐÞÊÎÑо¿ÖС£¡£¡£

tMeRIP-SeqÊÖÒÕÔÀíͼ£º£º£ºÍ¨¹ýTn5Ô¤ÏȼÓÌÖÂÛµÄRNAƬ¶Ï¾ÓÉͨÀýÃâÒß¹²³Áµíºó£¬£¬Ö±½ÓPCR¼´¿É»ñµÃ²âÐòÎĿ⡣¡£¡£
¿ËÈÕ£¬£¬¸ÃÑо¿Ð§¹û¡°Establishment of Transposase-assisted Low-input m6A Sequencing Technique ¡±½ÒÏþÔÚÆÚ¿¯Journal of Genetics and GenomicsÉÏ¡£¡£¡£ngµç×ÓÓÎÏ·ÉúÃü¿ÆÑ§Ñ§Ôº²©Ê¿ºó³ÂÌκͲ©Ê¿Ñо¿ÉúÀîÑÔΪ¸ÃÂÛÎÄÅäºÏµÚÒ»×÷Õߣ¬£¬Âæ¹ÛÕý½ÌÊÚΪͨѶ×÷Õß¡£¡£¡£Ïà¹ØÊÂÇé»ñµÃÖØµãÑз¢ÍýÏë¡¢¡¢¹ú¼Ò×ÔÈ»¿ÆÑ§»ù½ð¡¢¡¢¹ã¶«Ê¡×ÔÈ»¿ÆÑ§»ù½ðºÍÖйú²©Ê¿ºó¿ÆÑ§»ù½ðµÈÏîÄ¿×ÊÖú¡£¡£¡£
ÂÛÎÄÁ´½Ó£º£º£ºhttps://doi.org/10.1016/j.jgg.2021.08.007